Omidvar Farhadian
Abstract
In this research, gut contents of white leg shrimp Litopenaeus vannamei were investigated for 113 days culture period at earthen ponds in Delvar of Bushehr, by sampling every 15 days. Results showed that artificial food had highest weight percentage in gut contents (foregut, midgut, and hindgut) following ...
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In this research, gut contents of white leg shrimp Litopenaeus vannamei were investigated for 113 days culture period at earthen ponds in Delvar of Bushehr, by sampling every 15 days. Results showed that artificial food had highest weight percentage in gut contents (foregut, midgut, and hindgut) following in order by detritus, zooplankton, phytoplankton, and macro-benthic. In the beginning of culture period, macro-bentic were observed in larval gut contents while other food items were throughout culture period. The maximum occurrence of detritus in gut contents was at 26 and 40 days-old larvae while highest phytoplankton was obtained at 86-days-old larvae in end of culture period. The zooplankton had approximately similar amounts throughout culture period in different parts of gut, while macro-benthic only observed at 26-old-days larvae with maximum amount at foregut and midgut. The mean highest gut fullness attained at foregut (0.95 %) and lowest at hindgut (0.20 %). The dominant color of gut content was green-brown in all parts of gut that could be due to consumption of natural pond products. This study illustrated that most consumed food items in L. vannamei was prepared by natural pond foods. In addition, detritus and plankton have most important in L. vannamei during culture period.
Amir Jaferian; Hosein Zolgharnein; Mehdi Mohammadi; Mohammad Ali Salari Ali Abadi; Seyyed Javad Hoseini
Volume 12, Issue 1 , April 2013, , Pages 29-37
Abstract
Fourfinger Threadfin in shallow coastal waters with sandy or muddy bottom and into the lives of the rivers. The fish in the coastal areas of tropical and subtropical areas of the environment. The family of 7 genera and 35 species have been identified in the warm waters. Persian Gulf fish are apart from ...
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Fourfinger Threadfin in shallow coastal waters with sandy or muddy bottom and into the lives of the rivers. The fish in the coastal areas of tropical and subtropical areas of the environment. The family of 7 genera and 35 species have been identified in the warm waters. Persian Gulf fish are apart from the economic elite. Sampled using boats equipped with fishing hooks gill net and sometimes in the Persian Gulf was. And was amplified using the number six position. The highest number of alleles observed in the samples and place Eletet2 Bushehr (5 alleles) and the lowest in the province is the place Eletet16.0 and the samples (one allele). Ho Eletet16.0 samples collected in the range of the lowest position of the province and the province is the highest position Eletet16.0. Shannon index Eletet2 highest position in the province) 1.494 (and its lowest position in the province Eletet17 (0.637) is. AMOVA tests based on risk level 0.01 differences between samples (%76), the difference between the groups (regions) (%0), differences between regions (%24) was calculated. similar genetic criteria Nei, (1972) The province of Khuzestan and Bushehr specimens (0.486) and genetic distance between samples of Khuzestan and Bushehr (0.615) exists. In the present study, heterozygosity at the highest position in the province of Bushehr is Eletet2. The highest observed heterozygosity (Ho), 0.923 is the position Eletet16.0 and Bushehr specimens. The amount of heterozygosity observed in this study is more indicative of the amount expected heterozygosity genetic variability due to fish migration in the near and far and free access to water (high gene flow) is. The results suggest that it is probably a fish population in the areas of Fourfinger Threadfin conventional fisheries management perspective there is any, is the same.
Soad Yaish-Bachari; Hossein Zolgharnein; Mehdi Mohammadi; Mohammad Ali Salari-AliAbadi; Seied Ahmad Ghasemi
Volume 11, Issue 3 , November 2012, , Pages 62-70
Abstract
We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by ...
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We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by proteinase-K digestion, phenol: chloroform: isoamyl alcohol extraction and ethanol precipitation. The quality and quantity of DNA was assessed using 1% agarose gel electrophoresis and spectrophotomeric method. Polymerase chain reactions (PCR) were conducted on the target DNA using 6 primers RAPD. PCR products were separated by electrophoresis on polyacrylamid gels (8%) that were stained using silver nitrate. Allele frequency, the effective and real allele, expected and observed heterozygosis, genetic diversity, Shannon Information index, Gst and gene based on analysis of molecular variance (AMOVA) were calculated using the GENALEX and Pop Gene software. The average of Shannon index for Hendijan, Khore zangi and Delvar was respectively 0.3887, 0.2860 and 0.3509. Highest of Gst observed between Hendijan and Khore zangi (0.260) and lowest between Khore zangi and Delvar (0.195). Highest of gene flow (Nm) was observed between Delvar and Khore zangi (1.033) and lowest between Hendijan and Khore zangi (0.687). Highest of Genetic identity observed between Delvar and Khore zangi (0.924) but lowest was between Hendijan and Khore zangi community (0.833). Eventually this study show that three community of our research are separate and belong to two clusters.